C14orf169/NO66 Antibody

SKU: orb19772

Description

Goat polyclonal antibody to C14orf169

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:64000, WB: 0.3-1 μg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 70kDa band observed in lysates of cell line A431 (calculated MW of 71.1kDa according to NP_078920.2). Recommended concentration: 0.3-1 μg/ml. An additional band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: C14orf169 / NO66
• Protein Sequence: GAQHSDSKDPR
• Molecular Weight: 71.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti C14orf169 antibody, anti NO66 antibody, anti chromosome 14 open reading frame 169 antibody, anti FLJ21802 antibody, anti MAPJD antibody

orb19772 (0.3ug/ml) staining of A431 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

C14orf169 / NO66

NCBI Gene ID

79697