Goat anti-Bruno-like 5 Antibody

• Catalog Number: orb19642
• Category: Antibodies
• Description: Goat polyclonal antibody to BRUNOL5
• Target: Bruno-like 5
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: EPPGGQPDGMKD
• RRID: AB_10752492
• MW: 52.4
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:1000, WB: 1-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:1000.WB: Approx 55kDa band observed in Human Heart lysates (calculated MW of 52.4kDa according to NP_068757.2). Recommended concentration: 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti BRUNOL5 antibody, anti bruno-like 5, RNA bind
• Note: For research use only
• Entrez: 60680

orb19642 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.