BLNK/SLP-65 Antibody

• Catalog Number: orb18354
• Category: Antibodies
• Description: Goat polyclonal antibody to BLNK
• Target: BLNK / SLP-65
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KDSTRLKYAVKVS
• RRID: AB_10755391
• MW: 50.5; 48.2; 44.5; 42.2; 33.0
• Tested applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 10ug/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 70kDa band observed in lysates of cell line Daudi (calculated MW of 50.5kDa according to 037446.1). The observed molecular weight corresponds to how SLP-65 is expected to run. However, when transiently expressed in HEK293, a 50kDa band is observed. Recommended concentration: 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti BLNK antibody, anti SLP-65 antibody, anti B-c
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 29760
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Daudi lysate using BLNK antibody

Western blot analysis of HEK293 cells using BLNK antibody

Immunofluorescence analysis of HepG2 cells using BLNK antibody

Flow cytometric analysis of Daudi cells using BLNK antibody