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Catalog Number | orb18354 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to BLNK |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IF, WB |
Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
Dilution range | ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 10ug/ml |
Conjugation | Unconjugated |
MW | 50.5; 48.2; 44.5; 42.2; 33.0 |
Target | BLNK / SLP-65 |
Entrez | 29760 |
Protein Sequence | KDSTRLKYAVKVS |
RRID | AB_10755391 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti BLNK antibody, anti SLP-65 antibody, anti B-c Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 70kDa band observed in lysates of cell line Daudi (calculated MW of 50.5kDa according to 037446.1). The observed molecular weight corresponds to how SLP-65 is expected to run. However, when transiently expressed in HEK293, a 50kDa band is observed. Recommended concentration: 1-3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Daudi lysate using BLNK antibody
Western blot analysis of HEK293 cells using BLNK antibody
Immunofluorescence analysis of HepG2 cells using BLNK antibody
Flow cytometric analysis of Daudi cells using BLNK antibody