BIN1 Antibody

• Catalog Number: orb19949
• Category: Antibodies
• Description: Goat polyclonal antibody to BIN1
• Target: BIN1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: SDNAPAKGNKSPS
• RRID: AB_10768222
• MW: 45.6; 64.7; 59.9; 55.2; 54.9; 56.5; 53.0; 51.7
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:1000, WB: 1-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:1000.WB: Experiments gave bands at approx 70kDa and 60kDa in Human Skeletal Muscle lysates after 0.1 μg/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Chang et al, Cancer Res. 2007 Jan 1;67(1):100-7.; PMID: 17210688). This protein has a calculated MW of 64.7kDa according to NP_647593.1. Recommended concentration: 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti BIN1 antibody, anti bridging integrator 1 ant
• Research Area: Developmental Biology, Epigenetics, Infectious Dis
• Note: For research use only
• Entrez: 274

orb19949 (0.1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence