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Catalog Number | orb18342 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to SH3GLB1 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IHC, WB |
Reactivity | Bovine, Canine, Human, Mouse, Rat |
Dilution range | ELISA: 1:32000, WB: 0.3-1.0 μg/ml, IHC-P: 2.5 μg/ml |
Conjugation | Unconjugated |
MW | 40.8; 44.3; 43.3; 29.3 |
Target | BIF-1 / SH3GLB1 |
Entrez | 51100 |
Protein Sequence | QKGKVPITYLELLN |
RRID | AB_10752844 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti SH3GLB1 antibody, anti Bif-1 antibody, anti S Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 38kDa band observed in lysates of Human T cell line Jurkat and in Human Lung and Placenta (calculated MW of 40.8kDa according to NP_057093.1). Recommended concentration: 0.3-1.0 μg/ml. A minor band of unknown identity was also consistently observed at 50kDa in Jurkat and Placenta. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Jurkat lysate using SH3GLB1 antibody
Immunohistochemical staining of Human Colon using SH3GLB1 antibody