Goat anti-BID Antibody

• Catalog Number: orb18994
• Category: Antibodies
• Description: Goat polyclonal antibody to BID
• Target: BID
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: TYVRSLARNGMD
• RRID: AB_10755547
• MW: 26.8; 22.0; 11.3
• Tested applications: ELISA, IF, WB
• Dilution range: ELISA: 1:8000, WB: 0.1-0.3 μg/ml, IHC-P: 10 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 26+24kDa bands observed in Human fibroblast A431 lysates (calculated MW of 26.8kDa according to NP_932070.1 and 22.0kDa according to NP_001187.1). Recommended concentration: 0.1-0.3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti Apoptic death agonist antibody, anti BH3 inte
• Research Area: Apoptotic, Cancer, Epigenetics, Neuroscience, Sign
• Note: For research use only
• Entrez: 637
• Expiration Date: 12 months from date of receipt.

orb18994 (0.1ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunofluorescence analysis of MCF7 cells using BID antibody