Goat anti-BID Antibody

SKU: orb18994

Description

Goat polyclonal antibody to BID

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution range: ELISA: 1:8000, WB: 0.1-0.3 μg/ml, IHC-P: 10 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 26+24kDa bands observed in Human fibroblast A431 lysates (calculated MW of 26.8kDa according to NP_932070.1 and 22.0kDa according to NP_001187.1). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: BID
• Molecular Weight: 26.8; 22.0; 11.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti Apoptic death agonist antibody, anti BH3 interacting domain death agonist antibody, anti BH3 interacting domain death agonist p11 antibody, anti BH3 interacting domain death agonist p13 antibody, anti BH3 interacting domain death agonist p15 antibody, anti BH3-interacting domain death agonist p11 antibody, anti BID antibody, anti BID isoform ES(1b) antibody, anti BID isoform L(2) antibody, anti BID isoform Si6 antibody, anti Desmocollin type 4 antibody, anti FP497 antibody, anti p11 BID antibody, anti p13 BID antibody, anti p15 BID antibody, anti p22 BID antibody

orb18994 (0.1ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunofluorescence analysis of MCF7 cells using BID antibody

Quick Database Links

Gene Symbol

BID

NCBI Gene ID

637