BAZ2B Antibody

SKU: orb18883

Description

Goat polyclonal antibody to BAZ2B

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:4000, WB: 0.3 μg/ml, IHC-P: 1-2µg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.IHC: In paraffin embedded Human Small Intestine shows primarily nuclear staining in a number of cells of the lamina propria. Recommended concentration, 1-2ug/ml.WB: Preliminary experiments gave an approx 35kDa band in Testis and Brain lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 221kDa according to NP_038478. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: BAZ2B
• Protein Sequence: YFEKKWTDTFKVS
• Molecular Weight: 240
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti BAZ2B antibody, anti bromodomain adjacent to zinc finger domain, 2B antibody, anti DKFZP434H071 antibody, anti DKFZp762I0516 antibody, anti FLJ45644 antibody, anti WALp4 antibody

orb18883 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

BAZ2B

NCBI Gene ID

29994