BAF53A and BAF53B Antibody

• Catalog Number: orb18766
• Category: Antibodies
• Description: Goat polyclonal antibody to ACTL6A / ACTL6B
• Target: BAF53A and BAF53B
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Porcine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: YEEGGKQCVERKCP
• RRID: AB_10767383
• MW: 47.5; 43.2; 46.9
• Tested applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:16000, WB: 0.5-2μg/ml, IF/ICC: 10μg/ml, FACS: 10ug/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 48kDa band observed in Hela lysates (predicted MW of 49kDa according to NP_057272, NP_004292). Recommended for use at 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti BAF53A antibody, anti BAF53B antibody, anti A
• Research Area: Neuroscience
• Note: For research use only
• Entrez: 86, 51412

Flow cytometric analysis of paraformaldehyde fixed A431 cells using ACTL6A / ACTL6B antibody.

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells using ACTL6A / ACTL6B antibody.

Immunofluorescence analysis of paraformaldehyde fixed A431 cells using ACTL6A / ACTL6B antibody.

Western blot analysis of NIH3T3 nuclear cell lysate using ACTL6A / ACTL6B antibody.

Western blot analysis of Rat Skeletal Muscle lysate using ACTL6A / ACTL6B antibody.