BAF53A and BAF53B Antibody

SKU: orb18766

Description

Goat polyclonal antibody to ACTL6A / ACTL6B

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: ELISA: 1:16000, WB: 0.5-2μg/ml, IF/ICC: 10μg/ml, FACS: 10ug/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Porcine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 48kDa band observed in Hela lysates (predicted MW of 49kDa according to NP_057272, NP_004292). Recommended for use at 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: BAF53A and BAF53B
• Protein Sequence: YEEGGKQCVERKCP
• Molecular Weight: 47.5; 43.2; 46.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti BAF53A antibody, anti BAF53B antibody, anti ACTL6 antibody, anti BAF53a antibody, anti BAF53b antibody, anti ACTL6A antibody, anti ACTL6B antibody, anti 53 kDa BRG1-associated factor B antibody, anti Arp4 antibody, anti INO80K antibody, anti MGC5382 antibody, anti BAF complex 53 kDa subunit antibody, anti BAF53 antibody, anti BRG1-associated factor antibody, anti INO80 complex subunit K antibody, anti hArpN beta antibody

Flow cytometric analysis of paraformaldehyde fixed A431 cells using ACTL6A / ACTL6B antibody.

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells using ACTL6A / ACTL6B antibody.

Immunofluorescence analysis of paraformaldehyde fixed A431 cells using ACTL6A / ACTL6B antibody.

Western blot analysis of NIH3T3 nuclear cell lysate using ACTL6A / ACTL6B antibody.

Western blot analysis of Rat Skeletal Muscle lysate using ACTL6A / ACTL6B antibody.

Quick Database Links

Gene Symbol

BAF53A and BAF53B

NCBI Gene ID

51412, 86