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Goat anti-B5 Receptor / PCID1 / EIF3M Antibody

Catalog Number: orb19106

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb19106
CategoryAntibodies
DescriptionGoat polyclonal antibody to EIF3M
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, IHC
ReactivityBovine, Canine, Human, Mouse, Rat
Dilution rangeELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 5-10µg/ml
ConjugationUnconjugated
MW42.5
TargetB5 Receptor / PCID1 / EIF3M
Entrez10480
Protein SequenceNAWKQNLNKVKN
RRIDAB_10749622
StorageAliquot and store at -20°C. Minimize freezing and thawing.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Alternative namesanti B5 receptor antibody, anti PCID1 antibody, an
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NoteFor research use only
Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Testis shows strong staining of cytoplasm in select spermatocytes. Recommended concentration, 5-10ug/ml.WB: Approx 38-40kDa band observed in lysates of human lung carcinoma cell line A549 (calculated MW of 42.5kDa according to NP_006351.2). Recommended concentration: 1-3 μg/ml.
Expiration Date12 months from date of receipt.
Goat anti-B5 Receptor / PCID1 / EIF3M Antibody

orb19106 (0.1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Goat anti-B5 Receptor / PCID1 / EIF3M Antibody

orb19106 (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.