B5 Receptor/PCID1/EIF3M Antibody

• Catalog Number: orb19106
• Category: Antibodies
• Description: Goat polyclonal antibody to EIF3M
• Target: B5 Receptor / PCID1 / EIF3M
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: NAWKQNLNKVKN
• RRID: AB_10749622
• MW: 42.5
• Tested applications: ELISA, IHC
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 5-10µg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Testis shows strong staining of cytoplasm in select spermatocytes. Recommended concentration, 5-10ug/ml.WB: Approx 38-40kDa band observed in lysates of human lung carcinoma cell line A549 (calculated MW of 42.5kDa according to NP_006351.2). Recommended concentration: 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti B5 receptor antibody, anti PCID1 antibody, an
• Note: For research use only
• Entrez: 10480

orb19106 (0.1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19106 (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.