You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb19106 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to EIF3M |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC |
Reactivity | Bovine, Canine, Human, Mouse, Rat |
Dilution range | ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 5-10µg/ml |
Conjugation | Unconjugated |
MW | 42.5 |
Target | B5 Receptor / PCID1 / EIF3M |
Entrez | 10480 |
Protein Sequence | NAWKQNLNKVKN |
RRID | AB_10749622 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
Alternative names | anti B5 receptor antibody, anti PCID1 antibody, an Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Testis shows strong staining of cytoplasm in select spermatocytes. Recommended concentration, 5-10ug/ml.WB: Approx 38-40kDa band observed in lysates of human lung carcinoma cell line A549 (calculated MW of 42.5kDa according to NP_006351.2). Recommended concentration: 1-3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb19106 (0.1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb19106 (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.