B5 Receptor/PCID1/EIF3M Antibody

SKU: orb19106

Description

Goat polyclonal antibody to EIF3M

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 5-10µg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Testis shows strong staining of cytoplasm in select spermatocytes. Recommended concentration, 5-10ug/ml.WB: Approx 38-40kDa band observed in lysates of human lung carcinoma cell line A549 (calculated MW of 42.5kDa according to NP_006351.2). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: B5 Receptor / PCID1 / EIF3M
• Protein Sequence: NAWKQNLNKVKN
• Molecular Weight: 42.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti B5 receptor antibody, anti PCID1 antibody, anti PCI domain containing 1 (herpesvirus entry mediator) antibody, anti FLJ29030 antibody, anti GA17 antibody, anti hfl-B5 antibody, anti dendritic cell protein antibody, anti fetal lung protein B5 antibody, anti EIF3M antibody, anti eukaryotic translation initiation factor 3, subunit M antibody, anti B5 antibody, anti PCID1 antibody

orb19106 (0.1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19106 (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

B5 Receptor / PCID1 / EIF3M

NCBI Gene ID

10480