B3GNT2 Antibody

• Catalog Number: orb19651
• Category: Antibodies
• Description: Goat polyclonal antibody to B3GNT2
• Target: B3GNT2
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: EKHKGFRTFDIE
• RRID: AB_10753577
• MW: 46
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 45kDa band observed in Human Pancreas lysates (calculated MW of 46.0kDa according to NP_006568.2). Recommended concentration: 0.1-0.3 μg/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti B3GNT2 antibody, anti UDP-GlcNAc:betaGal beta
• Note: For research use only
• Entrez: 10678

orb19651 (0.1ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.