Atrial natriuretic factor Antibody

SKU: orb125226

Description

Goat polyclonal antibody to NPPA.

Research Area

Cardiovascular Disease

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IHC, WB
Dilution Range	ELISA: 1:32000, WB: 0.01-0.03 μg/ml, IHC-P: 5-10µg/ml
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Canine, Porcine
Application Notes	WB: Approx 19kDa band observed in Mouse and Rat Heart lysates (calculated MW of 16.4kDa according to Human precursor NP_006163.1 and 16.6kDa accordiong to Mouse precursor NP_032751.1). Recommended concentration: 0.01-0.03µg/ml.. IHC: In paraffin embedded Human Heart shows vesicular staining inside the cardiac fibrils, accumulating towards the cell membrane. Recommended concentration, 5-10µg/ml. Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Atrial natriuretic factor
Protein Sequence	RIGAQSGLGCNSFR
Molecular Weight	16.4
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti ANF antibody, anti ANP antibody, anti ATFB6 antibody, anti atrial natriuretic factor antibody, anti atriopeptin antibody, anti cardionatrin antibody, anti CDD-ANF antibody, anti natriuretic peptide A antibody, anti natriuretic peptide precursor A antibody, anti PND antibody, anti prepronatriodilatin antibody

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Immunohistochemical staining of Human Heart using NPPA antibody

Western blot analysis of Mouse Heart lysate using NPPA antibody

Quick Database Links

Gene Symbol

Atrial natriuretic factor

NCBI Gene ID

4878

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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