Atoh7 (mouse) Antibody

SKU: orb19577

Description

Goat polyclonal to Atoh7

Research Area

Developmental Biology

Images & Validation

• Tested Applications: ELISA
• Dilution Range: ELISA: 1:32000, WB: 0.3 μg/ml
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave bands at approx 50kDa and 38kDa in Mouse Eye and Rat Optical Nerve lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 16.9kDa according to NP_058560.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Atoh7 (mouse)
• Protein Sequence: CEQRGRDHPYLP
• Molecular Weight: 16.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Atoh7 antibody, anti atonal homolog 7 (Drosophila) antibody, anti Math antibody, anti atonal homolog 7 antibody

Quick Database Links

Gene Symbol

Atoh7 (mouse)