ATF7 Antibody

SKU: orb18317

Description

Goat polyclonal antibody to ATF7

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:16000, WB: 0.5-2 μg/ml, IHC-P: 3-5µg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Testis shows staining of select spermatocytes in Human Testis. Recommended concentration, 3-5ug/ml.WB: Approx 35kDa band seen in HeLa cell lysates. Recommended for use at 0.5-2 μg/ml. Please note the band size is in agreement with previous experimental data (J Biol Chem 2001 Apr 27;276(17):13718-26).

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ATF7
• Molecular Weight: 53.0; 51.8; 49.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti ATF7 antibody, anti ATFA antibody, anti activating transcription factor 7 antibody, anti MGC57182 antibody

orb18317 staining (0.5ug/ml) of HeLa lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18317 (3.8ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

ATF7

NCBI Gene ID

11016