Goat anti-ATF6 (aa437-449) Antibody

• Catalog Number: orb107677
• Category: Antibodies
• Description: Goat polyclonal antibody to ATF6
• Target: ATF6 (aa437-449)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Human, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: NSYRYDHSVSNDK
• MW: 74.6
• Tested applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:128000, WB: 0.3-2 μg/ml, IHC-P: 10ug/ml
• Application notes: WB: Approx 75-80kDa band observed in lysates of cell lines HeLa and MCF7, and in HeLa and Jurkat nuclear cell lysates (calculated MW of 74.6kDa according to NP_031374.2). Recommended concentration: 0.3-2µg/ml. Primary incubation 1 hour at room temperature.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti-Activating transcription factor 6 alpha antib
• Note: For research use only
• Entrez: 22926

Western blot analysis of HeLa (Lane 1) and MCF7 (Lane 2) cell lysate, and HeLa (Lane 3) and Jurkat (Lane 4) nuclear cell lysate using ATF6 antibody

Immunofluorescence analysis of A431 cells, permeabilized with 0.15% Triton of ATF6 antibody

Immunofluorescence analysis of HeLa cells, permeabilized with 0.15% Triton of ATF6 antibody

Flow cytometric analysis of A431 cells using ATF6 antibody