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Ascl3 (mouse) Antibody

SKU: orb125133

Description

Goat polyclonal antibody to Ascl3

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:8000, WB: 0.1-0.3 μg/ml, IHC-P: 3.75 μg/ml
ReactivityHuman, Mouse
Predicted ReactivityBovine
Application Notes
WB: Approx 23kDa band observed in Mouse Liver lysates (calculated MW of 20.2kDa according to NP_064435.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.. IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75µg/ml.

Key Properties

ClonalityPolyclonal
TargetAscl3 (mouse)
Protein SequenceHLPEDYLEKRLSK
Molecular Weight20.2
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti Ascl3 antibody, anti achaete-scute complex homolog 3 (Drosophila) antibody, anti MGC129412 antibody, anti MGC129413 antibody, anti Sgn1 antibody, anti bHLHa42 antibody, anti ASH-3 antibody, anti ICRFP703B1614Q5.4 antibody, anti ICRFP703N2430Q5.4 antibody, anti achaete-scute homolog 3 antibody, anti bHLH transcriptional regulator Sgn-1 antibody, anti mASH-3 antibody, anti mASH3 antibody

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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