Ascl3 (mouse) Antibody

• Catalog Number: orb125133
• Category: Antibodies
• Description: Goat polyclonal antibody to Ascl3
• Target: Ascl3 (mouse)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: HLPEDYLEKRLSK
• MW: 20.2
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:8000, WB: 0.1-0.3 μg/ml, IHC-P: 3.75 μg/ml
• Application notes: WB: Approx 23kDa band observed in Mouse Liver lysates (calculated MW of 20.2kDa according to NP_064435.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.. IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75µg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti Ascl3 antibody, anti achaete-scute complex ho
• Note: For research use only
• Entrez: 56676

Western blot analysis of Mouse Liver lysate using Ascl3 antibody