ASCL1 (aa79-91) Antibody

SKU: orb2001

Description

Goat polyclonal antibody to ASCL1

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution Range: ELISA: 1:32000, WB: 0.3-1 μg/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  WB: Approx 28kDa band observed in Mouse and Rat Lung lysates (calculated MW of 24.7kDa according to Mouse NP_032579.2 and 24.9kDa according to Rat NP_071779) . Recommended concentration: 0.3-1µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10µg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ASCL1 (aa79-91)
• Protein Sequence: HKSAPKQVKRQRS
• Molecular Weight: 25.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ASCL1; achaete-scute complex homolog 1 (Drosophila); ASH1; HASH1; MASH1; bHLHa46; ASH-1; achaete scute protein; achaete-scute complex-like 1; achaete-scute homolog 1; class A basic helix-loop-helix protein 46

Western blot analysis of Mouse and Rat Lung lysate using ASCL1 antibody

Quick Database Links

Gene Symbol

ASCL1 (aa79-91)

NCBI Gene ID

429