AS160/TBC1D4 Antibody

SKU: orb19407

Description

Goat polyclonal antibody to TBC1D4

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 3-5µg/ml
• Reactivity: Human
• Predicted Reactivity: Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Skeletal Muscle shows striated pattern staining of majority of muscular fibres. Recommended concentration, 3-5ug/ml.WB: Approx 150kDa band observed in lysates of cell lines Daudi and MOLT4 (calculated MW of 147kDa according to NP_055647.2). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: AS160 / TBC1D4
• Molecular Weight: 147
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti TBC1D4 antibody, anti AS160 antibody, anti TBC1 domain family, member 4 antibody, anti RP11-159J2.1 antibody, anti DKFZp779C0666 antibody, anti Acrg embryonic lethality (mouse) minimal region ortholog antibody, anti OTTHUMP00000040818 antibody, anti TBC (Tre-2, BUB2, CDC16) domain-containing protein antibody, anti KIAA0603 antibody

orb19407 (0.1ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19407 (3.8ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

AS160 / TBC1D4

NCBI Gene ID

9882