Goat anti-AS160 / TBC1D4 Antibody

• Catalog Number: orb19407
• Category: Antibodies
• Description: Goat polyclonal antibody to TBC1D4
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Canine, Human
• Dilution range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 3-5µg/ml
• Conjugation: Unconjugated
• MW: 147
• Target: AS160 / TBC1D4
• Entrez: 9882
• Protein Sequence: DDPEKIEERKKSK
• RRID: AB_10748770
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti TBC1D4 antibody, anti AS160 antibody, anti TB
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Skeletal Muscle shows striated pattern staining of majority of muscular fibres. Recommended concentration, 3-5ug/ml.WB: Approx 150kDa band observed in lysates of cell lines Daudi and MOLT4 (calculated MW of 147kDa according to NP_055647.2). Recommended concentration: 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19407 (0.1ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19407 (3.8ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.