Goat anti-Arylsulfatase D Antibody

SKU: orb19805

Description

Goat polyclonal to Arylsulfatase D

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:16000, WB: 0.3-1 μg/ml
• Reactivity: Human
• Application Notes:
  This antibody is expected to recognise both reported isoforms (NP_001660.2; NP_033667.2)ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 60+40kDa bands observed in lysates of cell lines A549 and HepG2 (calculated MW of 64.9kDa according to NP_001660.2 and of 42.3 according to NP_033667.2). Recommended concentration: 0.3-1 μg/ml. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Arylsulfatase D
• Protein Sequence: DASNGYRALQWNA
• Molecular Weight: 64.9; 42,3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti arylsulfatase D antibody, anti ARSD antibody

orb19805 (0.3ug/ml) staining of A459 lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Arylsulfatase D

NCBI Gene ID

414