ARPC2 Antibody

• Catalog Number: orb18679
• Category: Antibodies
• Description: Goat polyclonal antibody to ARPC2
• Target: ARPC2
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: EMKTITGKTFSSR
• RRID: AB_10750733
• MW: 34.3
• Tested applications: ELISA, IF, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.1-0.5 μg/ml, IHC-P: 10 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Spleen shows cytoplasm staining in some splenocytes in and around a T-cell area. Recommended concentration, 3-5ug/ml.WB: Approx 33-34kDa band observed in Hela and Human Skeletal Muscle lysates (predicted MW of 34kDa according to NP_005722). Recommended for use at 0.1-0.5 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti ARPC2 antibody, anti actin related protein 2/
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 10109
• Expiration Date: 12 months from date of receipt.

orb18679 staining (0.2ug/ml) of Hela lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18679 (3.8ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western blot analysis of CACO-2 (A), (0.1ug/ml) HeLa (B) and Jurkat (C) lysate using ARPC2 antibody

Immunofluorescence analysis of A431 cells using ARPC2 antibody

Immunofluorescence analysis of HeLa cells using ARPC2 antibody