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Goat anti-ARPC2 Antibody

Catalog Number: orb18679

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb18679
CategoryAntibodies
DescriptionGoat polyclonal antibody to ARPC2
TargetARPC2
ClonalityPolyclonal
Species/HostGoat
ConjugationUnconjugated
ReactivityBovine, Canine, Human, Mouse, Rat
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Protein SequenceEMKTITGKTFSSR
RRIDAB_10750733
MW34.3
Tested applicationsELISA, IF, IHC, WB
Dilution rangeELISA: 1:32000, WB: 0.1-0.5 μg/ml, IHC-P: 10 μg/ml
Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Spleen shows cytoplasm staining in some splenocytes in and around a T-cell area. Recommended concentration, 3-5ug/ml.WB: Approx 33-34kDa band observed in Hela and Human Skeletal Muscle lysates (predicted MW of 34kDa according to NP_005722). Recommended for use at 0.1-0.5 μg/ml.
StorageAliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesanti ARPC2 antibody, anti actin related protein 2/
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NoteFor research use only
Entrez10109
Goat anti-ARPC2 Antibody

orb18679 staining (0.2ug/ml) of Hela lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Goat anti-ARPC2 Antibody

orb18679 (3.8ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Goat anti-ARPC2 Antibody

Western blot analysis of CACO-2 (A), (0.1ug/ml) HeLa (B) and Jurkat (C) lysate using ARPC2 antibody

Goat anti-ARPC2 Antibody

Immunofluorescence analysis of A431 cells using ARPC2 antibody

Goat anti-ARPC2 Antibody

Immunofluorescence analysis of HeLa cells using ARPC2 antibody