ARPC2 Antibody

SKU: orb18679

Description

Goat polyclonal antibody to ARPC2

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.1-0.5 μg/ml, IHC-P: 10 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Spleen shows cytoplasm staining in some splenocytes in and around a T-cell area. Recommended concentration, 3-5ug/ml.WB: Approx 33-34kDa band observed in Hela and Human Skeletal Muscle lysates (predicted MW of 34kDa according to NP_005722). Recommended for use at 0.1-0.5 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ARPC2
• Molecular Weight: 34.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti ARPC2 antibody, anti actin related protein 2/3 complex, subunit 2, 34kDa antibody, anti ARC34 antibody, anti PRO2446 antibody, anti p34-Arc antibody, anti PNAS-139 antibody, anti ARP2/3 protein compex subunit 34 antibody, anti actin related protein 2/3 complex subunit 2 antibody

orb18679 staining (0.2ug/ml) of Hela lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18679 (3.8ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western blot analysis of CACO-2 (A), (0.1ug/ml) HeLa (B) and Jurkat (C) lysate using ARPC2 antibody

Immunofluorescence analysis of A431 cells using ARPC2 antibody

Immunofluorescence analysis of HeLa cells using ARPC2 antibody

Quick Database Links

Gene Symbol

ARPC2

NCBI Gene ID

10109