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Catalog Number | orb18406 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to ARL2 |
Species/Host | Goat |
Tested applications | ELISA, FC, IF, WB |
Reactivity | Canine, Human |
Dilution range | ELISA: 1:1000, WB: 1-3 μg/ml |
Conjugation | Unconjugated |
Target | ARL2 |
Protein Sequence | LDDISSRIFTAD |
RRID | AB_10755421 |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | ARL2; ADP-ribosylation factor-like 2; ARFL2; ADP=r Read more... |
Note | For research use only |
Application notes | WB: Approx. 18kDa band observed in lysates of cell line A431 and approx. 20kDa in nuclear lysates of cell line HeLa (calculated MW of 20.9kDa according to NP_001658.2). Recommended concentration: 0.5-1µg/ml.Primary incubation 1 hour at room temperature.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration |
Expiration Date | 12 months from date of receipt. |
orb18406 staining (2ug/ml) of U937 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.