ARL2 Antibody

• Catalog Number: orb18406
• Category: Antibodies
• Description: Goat polyclonal antibody to ARL2
• Target: ARL2
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: LDDISSRIFTAD
• RRID: AB_10755421
• Tested applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:1000, WB: 1-3 μg/ml
• Application notes: WB: Approx. 18kDa band observed in lysates of cell line A431 and approx. 20kDa in nuclear lysates of cell line HeLa (calculated MW of 20.9kDa according to NP_001658.2). Recommended concentration: 0.5-1µg/ml.Primary incubation 1 hour at room temperature.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ARL2; ADP-ribosylation factor-like 2; ARFL2; ADP=r
• Research Area: Epigenetics
• Note: For research use only

orb18406 staining (2ug/ml) of U937 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.