ARIH1/HHARI Antibody

SKU: orb18430

Description

Goat polyclonal antibody to ARIH1

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.2-1 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 65kDa band seen in U937 lysate [Predicted MW of approx. 61kDa according to NP_005735]. Recommended for use at 0.2-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ARIH1 / HHARI
• Protein Sequence: HEGYEKDLWEYIED
• Molecular Weight: 64.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ARIH1 antibody, anti HHARI antibody, anti ARI antibody, anti HARI antibody, anti UBCH7BP antibody, anti ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila) antibody, anti ariadne homolog antibody, anti ariadne, Drosophila, homolog of antibody, anti DKFZp686O13120 antibody, anti FLJ20329 antibody, anti FLJ93118 antibody, anti ariadne ubiquitin-conjugating enzyme E2 binding protein homolog 1 antibody

orb18430 (5µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb18430 staining (0.5µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

ARIH1 / HHARI

NCBI Gene ID

25820