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Goat anti-Arginase, type 1 / arg1(rat) Antibody
Catalog Number: orb19491
| Catalog Number | orb19491 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal to arg1(rat) |
| Target | Arginase, type 1 / arg1(rat) |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Mouse, Rat |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Protein Sequence | NHKPETDYLKPPK |
| RRID | AB_10746439 |
| MW | 35 |
| Tested applications | IF, WB |
| Dilution range | ELISA: 1:32000, WB: 0.01.0.03 μg/ml |
| Application notes | Peptide with sequence C-NHKPETDYLKPPK, from the C Terminus of the protein.ELISA: antibody detection limit dilution 1:128000.WB: Approx 37kDa band observed in Mouse and Rat Liver lysates (calculated MW of 34.8kDa according to mouse NP_031508.1). Recommended concentration: 0.03-0.1µg/ml. |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti Arg1 antibody, anti arginase 1 antibody, anti Read more... |
| Note | For research use only |
1 μg/ml staining of Mouse (A) and (2 μg/ml Rat (B) Liver lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.
Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
