Goat anti-Arginase, type 1 / arg1(rat) Antibody

SKU: orb19491

Description

Goat polyclonal to arg1(rat)

Images & Validation

• Tested Applications: IF, WB
• Dilution range: ELISA: 1:32000, WB: 0.01.0.03 μg/ml
• Reactivity: Mouse, Rat
• Application Notes:
  Peptide with sequence C-NHKPETDYLKPPK, from the C Terminus of the protein.ELISA: antibody detection limit dilution 1:128000.WB: Approx 37kDa band observed in Mouse and Rat Liver lysates (calculated MW of 34.8kDa according to mouse NP_031508.1). Recommended concentration: 0.03-0.1µg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Arginase, type 1 / arg1(rat)
• Molecular Weight: 35
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti Arg1 antibody, anti arginase 1 antibody, anti AI type I arginase antibody, anti arginase 1 liver antibody

1 μg/ml staining of Mouse (A) and (2 μg/ml Rat (B) Liver lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.

Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Quick Database Links

Gene Symbol

Arginase, type 1 / arg1(rat)