ARF4 (aa137-150) Antibody

• Catalog Number: orb12320
• Category: Antibodies
• Description: Goat polyclonal to ARF4.
• Target: ARF4 (aa137-150)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: SEMTDKLGLQSLRN
• MW: 20.5
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
• Application notes: WB: Approx 19kDa band observed in lysates of cell lines NIH3T3 and HeLa (calculated MW of 20.5kDa according to Human NP_001651.1 and 20.4kDa according to Mouse NP_031505.1 ). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Thymus. Recommended concentration: 5µg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti ARF4 antibody
• Note: For research use only
• Entrez: 378
• Expiration Date: 12 months from date of receipt.

orb12320 (0.5µg/ml) staining of NIH3T3 (A) and HeLa (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb12320 (5µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.