Goat anti-ARF1 (variants 1-4) Antibody

• Catalog Number: orb19359
• Category: Antibodies
• Description: Goat polyclonal antibody to ARF1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, WB
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:8000, WB: 1-3 μg/ml
• Conjugation: Unconjugated
• MW: 20.7
• Target: ARF1 (variants 1-4)
• Entrez: 378, 375, 377, 376
• Protein Sequence: EGLDWLSNQLRNQK
• RRID: AB_10752936
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti ARF1 antibody, anti ADP-ribosylation factor 1
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 18kDa band observed in lysates of cell lines HEK293, A431 and HepG2 (calculated MW of 20.7kDa according to NP_001019397.1; NP_001649.1; NP_001019398.1; NP_001019399.1). Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19359 (1ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.