Goat anti-APOL4 (C Terminus) Antibody

SKU: orb19903

Description

Goat polyclonal antibody to APOL4

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 37kDa band observed in Human Placenta lysates (calculated MW of 38.7kDa according to NP_085146.2). Recommended concentration: 0.3-1 μg/ml. Two additional bands of unknown identity were also consistently observed at 50kDa and at 70kDa. The band at 50kDa was successfully blocked by incubation with the immunising peptide. The other band at 70kDa however was not blocked by the immunizing peptide and it is therefore a non-specific signal. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported We call for caution when the antibody is used for other assays than Western blot.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: APOL4 (C Terminus)
• Protein Sequence: ENLNELTHIHQSLK
• Molecular Weight: 38.7; 39.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti apolipoprotein L4 antibody, anti APOL4 antibody, anti apolipoprotein L, 4 antibody, anti APOL-IV antibody, anti APOLIV antibody, anti OTTHUMP00000028937 antibody, anti apolipoprotein L-IV antibody

orb19903 (0.5ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Skeletal using APOL4 antibody

Quick Database Links

Gene Symbol

APOL4 (C Terminus)

NCBI Gene ID

80832