Goat anti-APOL4 (C Terminus) Antibody

• Catalog Number: orb19903
• Category: Antibodies
• Description: Goat polyclonal antibody to APOL4
• Target: APOL4 (C Terminus)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Protein Sequence: ENLNELTHIHQSLK
• RRID: AB_10752780
• MW: 38.7; 39.2
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 37kDa band observed in Human Placenta lysates (calculated MW of 38.7kDa according to NP_085146.2). Recommended concentration: 0.3-1 μg/ml. Two additional bands of unknown identity were also consistently observed at 50kDa and at 70kDa. The band at 50kDa was successfully blocked by incubation with the immunising peptide. The other band at 70kDa however was not blocked by the immunizing peptide and it is therefore a non-specific signal. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported We call for caution when the antibody is used for other assays than Western blot.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti apolipoprotein L4 antibody, anti APOL4 antibo
• Note: For research use only
• Entrez: 80832

orb19903 (0.5ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Skeletal using APOL4 antibody