Goat anti-APOB Antibody

SKU: orb19937

Description

Goat polyclonal antibody to APOB

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 2 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Bands corresponding to the expected size of ApoB100 were detected in Human serum samples from five different individuals. Recommended concentration: 0.3-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: APOB
• Protein Sequence: TDLHLRYQKDKK
• Molecular Weight: 516
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti APOB antibody, anti apolipoprotein B (including Ag(x) antigen) antibody, anti FLDB antibody, anti apoB-100 antibody, anti apoB-48 antibody, anti apolipoprotein B antibody, anti apolipoprotein B48 antibody

orb19937 (0.5ug/ml) staining of Human Serum lysates. Data kindly provided by Yan Xie and NO Davidson, Washington USA. This antibody showed background below 150kDa in serum samples . We call for caution when used for other assays than Western blot.

orb19937 (2µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb19937 (2µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

APOB

NCBI Gene ID

338