APOA1BP Antibody

• Catalog Number: orb19953
• Category: Antibodies
• Description: Goat polyclonal antibody to Apolipoprotein AIBP
• Target: APOA1BP
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: PPYPDTECVYRLQ
• RRID: AB_10750436
• MW: 31.7
• Tested applications: ELISA, FC, WB
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:1000. Western blot: Approx. 27kDa band observed in lysates of cell line A431, and approx. 26kDa in lysates of cell line A549 and in Human Heart lysates (calculated MW of 31.7kDa according to NP_658985.2). Recommended concentration: 3µg/ml. Primary incubation 1 hour at room temperature. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti apolipoprotein A-I binding protein antibody,
• Note: For research use only
• Entrez: 128240

Primary incubation 1 hour at room temperature. Images A, B: A431 and A549 cell lysate at primary Ab concentration 3 ug/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Primary incubation 1 hour at room temperature. Image A: Human Heart lysate at primary Ab concentration 3 ug/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.