Goat anti-APBB1 / FE65 Antibody

• Catalog Number: orb19284
• Category: Antibodies
• Description: Goat polyclonal antibody to APBB1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Dilution range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml
• Conjugation: Unconjugated
• MW: 77.2; 77.0
• Target: APBB1 / FE65
• Entrez: 322
• Protein Sequence: GSLKPKRLGAHTP
• RRID: AB_10749112
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti APBB1 antibody, anti FE65 antibody, family B,
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 100kDa band observed in lysates of NIH/3T3 and HeLa (calculated MW of 77.2kDa according to NP_001155.1 and 77.0kDa according to NP_663722.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zambrano et al, J Biol Chem. 1998 Aug 7;273(32):20128-33.; PMID: 9685356). Recommended concentration: 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19284 (0.1ug/ml) staining of NIH/3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.