AP2A1 (aa706-727) Antibody

• Catalog Number: orb167689
• Category: Antibodies
• Description: Goat polyclonal antibody to AP2A1.
• Target: AP2A1 (aa706-727)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: ELEPPAPESPMALLADPAPAAD
• MW: 108; 105
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:128000, WB: 0.03-0.1 μg/ml
• Application notes: WB: Approx 110kDa band observed in Human Brain (Frontal cortex) lysates (calculated MW of 108kDa according to NP_055018.2). Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti AP2A1 antibody, anti adaptor-related protein
• Note: For research use only
• Entrez: 160

Western blot analysis of Human Frontal Cortex lysate (35ug protein in RIPA buffer) using AP2A1 antibody

Western blot analysis of Mouse Brain lysate (~5ug protein in SDSPAGE buffer) using AP2A1 antibody

Western blot analysis of HeLa cells using AP2A1 antibody

Immunohistochemical staining of Human Cortex using AP2A1 antibody