AP2A1 (aa706-727) Antibody

SKU: orb167689

Description

Goat polyclonal antibody to AP2A1.

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:128000, WB: 0.03-0.1 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Porcine, Rat
• Application Notes:
  WB: Approx 110kDa band observed in Human Brain (Frontal cortex) lysates (calculated MW of 108kDa according to NP_055018.2). Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: AP2A1 (aa706-727)
• Protein Sequence: ELEPPAPESPMALLADPAPAAD
• Molecular Weight: 108; 105
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti AP2A1 antibody, anti adaptor-related protein complex 2 antibody, anti alpha 1 subunit antibody, anti ADTAA antibody, anti AP2-ALPHA antibody, anti CLAPA1 antibody, anti 100 kDa coated vesicle protein A antibody, anti AP-2 complex subunit alpha-1 antibody, anti adapter-related protein complex 2 alpha-1 subunit antibody, anti adaptin antibody, anti alpha A antibody, anti adaptor protein complex AP-2 subunit alpha-1 antibody, anti alpha-adaptin A antibody, anti alpha1-adaptin antibody, anti clathrin assembly protein complex 2 alpha-A large chain antibody, anti clathrin-associated/assembly/adaptor protein antibody, anti large antibody, anti alpha 1 antibody, anti plasma membrane adaptor HA2/AP2 adaptin alpha A subunit antibody

Western blot analysis of Human Frontal Cortex lysate (35ug protein in RIPA buffer) using AP2A1 antibody

Western blot analysis of Mouse Brain lysate (~5ug protein in SDSPAGE buffer) using AP2A1 antibody

Western blot analysis of HeLa cells using AP2A1 antibody

Immunohistochemical staining of Human Cortex using AP2A1 antibody

Quick Database Links

Gene Symbol

AP2A1 (aa706-727)

NCBI Gene ID

160