Amisyn/STXBP6 Antibody

• Catalog Number: orb18598
• Category: Antibodies
• Description: Goat polyclonal to Amisyn
• Target: Amisyn / STXBP6
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: SAKSAISKEIFAP
• RRID: AB_10750725
• MW: 23.6
• Tested applications: ELISA, IHC
• Dilution range: ELISA: 1:32000, WB: 0.1 μg/ml, IHC-P: 3-5µg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Kidney shows cytoplasm staining of PCT and DCT cells Recommended concentration, 3-5ug/ml.WB: Preliminary experiments gave bands at both approx 25kDa and approx 45kDa in Human Brain extracts at 0.1 μg/ml. Please note that currently we cannot find an explanation in the literature for the 45kDa band we observe given the predicted size of approx. 24Kda according to NP_054897. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti STXBP6 antibody, anti syntaxin binding protei
• Research Area: Epigenetics, Neuroscience
• Note: For research use only
• Entrez: 29091

orb18598 (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.