Goat anti-AMID / PRG3 Antibody

SKU: orb18956

Description

Goat polyclonal antibody to AIFM2

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution range: ELISA: 1:128000, WB: 0.1 μg/ml, IHC-P: 4 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave an approx 48-50kDa band in Human Colon and Duodenum lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.5kDa according to NP_116186. The 48-50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: AMID / PRG3
• Molecular Weight: 40.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti AMID antibody, anti PRG3 antibody, anti apoptosis-inducing factor (AIF)-like mitochondrion-associated inducer of death antibody, anti 5430437E11Rik antibody, anti apoptosis-inducing factor (AIF)-homologous mitochondrion-associated inducer of death antibody, anti AIFM2 antibody, anti apoptosis-inducing factor, mitochondrion-associated, 2 antibody, anti RP11-367H5.2 antibody, anti OTTHUMP00000019740 antibody, anti apoptosis-inducing factor (AIF)-homologous mitochondrion-associated inducer of death antibody

Immunohistochemical staining of Human Heart using AIFM2 antibody

Immunohistochemical staining of Human Skeletal Muscle using AIFM2 antibody

Quick Database Links

Gene Symbol

AMID / PRG3

NCBI Gene ID

84883