ALS2CR2/ILPIP Antibody

• Catalog Number: orb18453
• Category: Antibodies
• Description: Goat polyclonal antibody to STRADB
• Target: ALS2CR2 / ILPIP
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: CDFPDEKDSYWEF
• RRID: AB_10749071
• MW: 47.0, 31.2
• Tested applications: ELISA, IF, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 3-5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: Immunohistochemistry: In paraffin embedded Human Heart shows staining of myocardial fibres in transverse section. Recommended concentration, 3-5ug/ml.WB: Approx 35kDa band observed in Human Heart, Liver and Muscle lysates (calculated MW of 47.0kDa according to NP_061041.2 (alpha isoform) and 31.2kDa according to AAF71042.1(beta isoform). In transfected HEK293 transiently expressing ILPIP a band of approx. 48kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti STRADB antibody, anti ALS2CR2 antibody, anti
• Research Area: Neuroscience
• Note: For research use only
• Entrez: 55437

HEK293 overexpressing ILPIP (RC203432) and probed with orb18453 (mock transfection in first lane), tested by Origene.

orb18453 (3.8ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb18453 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.