ALS2CR1/NIF3L1 Antibody

SKU: orb18452

Description

Goat polyclonal to ALS2CR1

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.2-1 μg/ml, IHC-P: 2-4µg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  This antibody is expected to recognise both reported isoforms. Variants (NP_001135827.1; NP_068596.2) encode the same isoform.ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Breast shows strong cytoplasm staining in epithelial and myoepithelial duct cells. Recommended concentration, 2-4ug/ml.WB: Approx 40kDa band seen in Human Heart and Human Testis extracts [Predicted MW of approx. 39kDa according to NP_068596]. Recommended for use at 0.2-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ALS2CR1 / NIF3L1
• Molecular Weight: 42.0; 39.0
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti NIF3L1 antibody, anti ALS2CR1 antibody, anti NIF3 NGG1 interacting factor 3-like 1 (S. pombe) antibody, anti CALS-7 antibody, anti MDS015 antibody, anti NIF3 (Ngg1 interacting factor 3, S.pombe homolog)-like 1 antibody, anti amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 1 antibody, anti NIF3 NGG1 interacting factor 3-like 1 antibody, anti OTTHUMP00000206190 antibody, anti OTTHUMP00000206191 antibody, anti OTTHUMP00000206192 antibody

orb18452 staining (1ug/ml) of Human Heart lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18452 (2.5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

ALS2CR1 / NIF3L1

NCBI Gene ID

60491