AKT1 Antibody

SKU: orb422482

Description

Goat polyclonal antibody to AKT1

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: ELISA: 1:32000, WB: 0.1-0.3ug/ml, IHC-P: 10 μg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  WB: Approx 60kDa band observed in MCF7 cell lysates (calculated MW of 55.7kDa according to NP_005154.2). This molecular weight is routinely observed by other sources. Recommended concentration:0.1-0.3ug/ml. Primary incubation 1 hour at room temperature.. Notes: Immunofluorescence: Strong expression of the protein seen in the microtubules/cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: AKT1
• Protein Sequence: QDVDQREAPLN
• Molecular Weight: 55.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AKT1; v-akt murine thymoma viral oncogene homolog 1 ; AKT; MGC99656; PKB; PRKBA; RAC; RAC-ALPHA ; RAC-alpha serine/threonine-protein kinase; murine thymoma viral (v-akt) oncogene homolog-1; protein kinase B; rac protein kinase alpha

0.3 μg/ml staining of MCF7 cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing Microtubule/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Quick Database Links

Gene Symbol

AKT1

NCBI Gene ID

207