Akt (phospho-S473) antibody

• Catalog Number: orb344403
• Category: Antibodies
• Description: Akt (phospho-S473) antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 17F6.B11
• Tested applications: ELISA, FC, IF, IHC, IP, Multiplex Assay, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: IgG1
• Immunogen: Anti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein, followed by hybridoma development.
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1:20,000, FC: User Optimized, IHC: 20 µg/mL, IF: 1:500-1:3,000, IP: User Optimized, WB: 1:500-1:3,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-AKT pS473 Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This phospho specific monoclonal antibody is specific for phosphorylated human and mouse AKT protein at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P31749
• NCBI: 62241011
• Storage: Store Anti-AKT pS473 (MOUSE) Monoclonal Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Alternative names: mouse anti-AKT pS473 Antibody, phospho AKT, RAC-PK
• Note: For research use only
• Application notes: Phospho AKT antibody is tested in ELISA, immunofluorescence, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required.
• Expiration Date: 12 months from date of receipt.

References

Deng, Dan et al. Silencing MEG3 protects PC12 cells from hypoxic injury by targeting miR-21 Artif Cells Nanomed Biotechnol, 48, 610-619 (2020)

PubMed: 32050796

Applications

WB

Reactivity

Rat

Wang, Weiwei et al. Notoginsenoside R1 alleviates high glucose-evoked damage in RSC96 cells through down-regulation of miR-503 Artif Cells Nanomed Biotechnol, 47, 3947-3954 (2019)

PubMed: 31581849

Applications

WB

Reactivity

Rat

Chen, Yanbo et al. Pretreatment of ghrelin protects H9c2 cells against hypoxia/reoxygenation-induced cell death via PI3K/AKT and AMPK pathways Artif Cells Nanomed Biotechnol, 47, 2179-2187 (2019)

PubMed: 31159591

Applications

WB

Reactivity

Rat

Li, Meng et al. Melatonin Inhibits the Ferroptosis Pathway in Rat Bone Marrow Mesenchymal Stem Cells by Activating the PI3K/AKT/mTOR Signaling Axis to Attenuate Steroid-Induced Osteoporosis Oxid Med Cell Longev, 2022, 8223737 (2022)

PubMed: 36035224

Applications

WB

Reactivity

Rat

Immunofluorescence analysis of A431 cells using Akt (phospho-S473) antibody

Immunofluorescence analysis of EGF treated A431 cells using Akt (phospho-S473) antibody

Immunofluorescence analysis of A431 cells using Akt (phospho-S473) antibody

Immunohistochemical staining of human prostate tissue using Akt (phospho-S473) antibody

Western blot analysis of NIH/3T3 cell lysates using Akt (phospho-S473) antibody

Western blot analysis of NIH/3T3 lysates using Akt (phospho-S473) antibody

Western blot analysis of A431 cells using Akt (phospho-S473) antibody

Western blot analysis of NIH 3T3 cells using Akt (phospho-S473) antibody