Goat anti-AKR1B10 Antibody

• Catalog Number: orb19743
• Category: Antibodies
• Description: Goat polyclonal antibody to AKR1B10
• Target: AKR1B10
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QSSHLEDYPFDAE
• RRID: AB_10751734
• MW: 36
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:8000, WB: 0.03-0.1 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 35kDa band observed in lysates of cell lines HEK293, A549 and HepG2 (calculated MW of 36.0kDa according to NP_064695.2). In transfected HEK293 transiently expressing AKR1B10 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti AKR1B10 antibody, anti aldo-keto reductase fa
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 57016

orb19743 (0.03ug/ml) staining of A549 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing AKR1B10 (RC203177) with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with orb19743 in the right panel (mock transfection in first and last lanes), tested by Origene.