AIP/ARA9 Antibody

• Catalog Number: orb19562
• Category: Antibodies
• Description: Goat polyclonal antibody to AIP
• Target: AIP / ARA9
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: EARIRQKDEEDKAR
• RRID: AB_10767395
• MW: 37.7
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:64000, WB: 0.02-0.06 μg/ml, IHC-P: 5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 37kDa band observed in lysates of cell line NIH/3T3 (calculated MW of 37.7kDa according to Human NP_003968.1 and 37.6kDa according to mouse NP_057875.1). In transfected HEK293 transiently expressing ARA9 a band of approx. 42kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.02-0.06 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti AIP antibody, anti ARA9 antibody, anti aryl h
• Research Area: Epigenetics, Neuroscience
• Note: For research use only
• Entrez: 9049
• Expiration Date: 12 months from date of receipt.

HEK293 overexpressing ARA9 (RC211157) and probed with orb19562 (mock transfection in first lane), tested by Origene.

Western blot analysis of NIH/3T3 cell lysate using AIP antibody

EB07776 (5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.