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AIFM1 Antibody

SKU: orb233664

Description

Goat polyclonal antibody to AIFM1

Research Area

Metabolism Research

Images & Validation

Tested ApplicationsELISA, IF, IHC, WB
Dilution RangeELISA: 1:128000, WB: 0.01-0.1 μg/ml, IHC-P: 5 μg/ml
ReactivityHuman, Mouse, Porcine, Rat
Predicted ReactivityBovine, Canine
Application Notes
This antibody is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1), isoform 3 (NP_665812.1) and isoform 4 (NP_001124318.1).ELISA: antibody detection limit dilution 1:128000.WB: Approx 70kDa band observed in lysates of cell line Jurkat (calculated MW of 66.9kDa according to NP_004199.1). Recommended concentration: 0.01-0.03µg/ml. Approx 70kDa band observed in Mouse and Rat Heart and Kidney lysates, and approx. 65kDa in Pig Heart lysates (calculated MW of 66.8kDa according to Mouse NP_036149, Rat NP_112646.1 and Pig NP_001284561.1). Recommended concentration: 0.01-0.1µg/ml.

Key Properties

ClonalityPolyclonal
TargetAIFM1
Protein SequenceNEVAKLFNIHED
Molecular Weight66.9; 66.3; 35.6; 28.4; 35.4
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti AIFM1 antibody, anti apoptosis-inducing factor antibody, anti mitochondrion-associated antibody, anti 1 antibody, anti AIF antibody, anti CMTX4 antibody, anti COWCK antibody, anti COXPD6 antibody, anti PDCD8 antibody, anti apoptosis-inducing factor 1 antibody, anti mitochondrial antibody, anti programmed cell death 8 (apoptosis-inducing factor) antibody, anti striatal apoptosis-inducing factor antibody

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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