Goat anti-AIFM1 Antibody

• Catalog Number: orb233664
• Category: Antibodies
• Description: Goat polyclonal antibody to AIFM1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:128000, WB: 0.01-0.1 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 66.9; 66.3; 35.6; 28.4; 35.4
• Target: AIFM1
• Entrez: 9131
• Protein Sequence: NEVAKLFNIHED
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti AIFM1 antibody, anti apoptosis-inducing facto
• Note: For research use only
• Application notes: This antibody is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1), isoform 3 (NP_665812.1) and isoform 4 (NP_001124318.1).ELISA: antibody detection limit dilution 1:128000.WB: Approx 70kDa band observed in lysates of cell line Jurkat (calculated MW of 66.9kDa according to NP_004199.1). Recommended concentration: 0.01-0.03µg/ml. Approx 70kDa band observed in Mouse and Rat Heart and Kidney lysates, and approx. 65kDa in Pig Heart lysates (calculated MW of 66.8kDa according to Mouse NP_036149, Rat NP_112646.1 and Pig NP_001284561.1). Recommended concentration: 0.01-0.1µg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Mouse, Rat and Pig Heart lysate using AIFM1 antibody

Western blot analysis of Jurkat lysate using AIFM1 antibody

Western blot analysis of Mouse (Lane 1) and Rat (Lane 2) Kidney lysate using AIFM1 antibody

Western blot analysis of NIH3T3 lysate using AIFM1 antibody