Goat anti-AIBZIP / CREB3L4 Antibody

• Catalog Number: orb18675
• Category: Antibodies
• Description: Goat polyclonal antibody to CREB3L4
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:32000, WB: 0.5-2 μg/ml, IHC-P: 2-4µg/ml
• Conjugation: Unconjugated
• MW: 43.4; 41.3
• Target: AIBZIP / CREB3L4
• Entrez: 148327
• Protein Sequence: PSGRIRSVLHADEM
• RRID: AB_10755573
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti AIBZIP antibody, anti cAMP responsive element
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Prostate shows strong staining of cytoplasm in the secretory cells of the gland. Recommended concentration, 2-4ug/ml.WB: Approx 50kDa band observed in Human Placenta lysate (predicted MW of 45kDa according to NP_570968). Recommended for use at 0.5-2 μg/ml.
• Expiration Date: 12 months from date of receipt.

2.5 µg/ml staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH6, AP-staining.

Primary incubation 1 hour at room temperature. Images A, B: MDA-MB-231, A431 cell lysate at primary Ab concentration 0.1 µg/ml, Image C: MCF7 cell lysate at primary Ab concentration 0.003 µg/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

Primary incubation 1 hour at room temperature. Images A, B: Human Kidney and Placenta lysate at primary Ab concentration 0.5 µg/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear, nuclear membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.