Goat anti-AGXT / AGT Antibody

• Catalog Number: orb20097
• Category: Antibodies
• Description: Goat polyclonal antibody to AGXT
• Target: AGXT / AGT
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DKAKKKMYSRK
• RRID: AB_10920891
• MW: 43
• Tested applications: ELISA, FC, IF
• Dilution range: ELISA: 1:2000, IHC-P: 10ug/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Preliminary experiments gave a faint 37kDa band in Human Liver lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 43.0kDa according to NP_000021.1. The 37kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti AGXT antibody, anti alanine-glyoxylate aminot
• Note: For research use only
• Entrez: 189
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using AGXT antibody.

Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells using AGXT antibody.

Flow cytometric analysis of paraformaldehyde fixed HepG2 cells using AGXT antibody.