AGRP/Agouti-related protein Antibody

SKU: orb19013

Description

Goat polyclonal antibody to AGRP

Research Area

Epigenetics, Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:32000, WB: 0.1 μg/ml, IHC-P: 0.05-0.1 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Porcine
• Application Notes:
  IHC: Frozen section of Human Hypothalamus shows staining of a group of neuronal cell bodies in the human infundibular (arcuate) nucleus and their varicose axons. Recommended concentration, 0.05-0.1μg/ml.WB: Preliminary experiments gave bands at approx 45kDa and 24kDa in Human Brain lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 14.4kDa according to NP_001129.1and NP_015531.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: AGRP / Agouti-related protein
• Protein Sequence: CRKLGTAMNPCSRT
• Molecular Weight: 14.4; 14.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti AGRP antibody, anti ART antibody, anti AGRT antibody, anti ASIP2 antibody, anti agouti related protein homolog (mouse) antibody, anti agouti (mouse) related protein antibody, anti Agouti-related transcript, mouse, homolog of antibody, anti MGC118963 antibody, anti agouti related protein homolog antibody

orb19013 (0.05µg/ml) staining of PFA-perfused cryosection of Human Hypothalamus. Antigen retrieval with citrate buffer Ph 6 at 80C for 30min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.

Quick Database Links

Gene Symbol

AGRP / Agouti-related protein

NCBI Gene ID

181