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Goat anti-AGRP / Agouti-related protein Antibody

Catalog Number: orb19013

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb19013
CategoryAntibodies
DescriptionGoat polyclonal antibody to AGRP
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, IHC
ReactivityCanine, Human, Porcine
Dilution rangeELISA: 1:32000, WB: 0.1 μg/ml, IHC-P: 0.05-0.1 μg/ml
ConjugationUnconjugated
MW14.4; 14.4
TargetAGRP / Agouti-related protein
Entrez181
Protein SequenceCRKLGTAMNPCSRT
RRIDAB_10749757
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesanti AGRP antibody, anti ART antibody, anti AGRT a
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NoteFor research use only
Application notesIHC: Frozen section of Human Hypothalamus shows staining of a group of neuronal cell bodies in the human infundibular (arcuate) nucleus and their varicose axons. Recommended concentration, 0.05-0.1μg/ml.WB: Preliminary experiments gave bands at approx 45kDa and 24kDa in Human Brain lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 14.4kDa according to NP_001129.1and NP_015531.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Expiration Date12 months from date of receipt.
Goat anti-AGRP / Agouti-related protein Antibody

orb19013 (0.05µg/ml) staining of PFA-perfused cryosection of Human Hypothalamus. Antigen retrieval with citrate buffer Ph 6 at 80C for 30min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.