Goat anti-Adenosine A1 receptor Antibody

• Catalog Number: orb19641
• Category: Antibodies
• Description: Goat polyclonal antibody to ADORA1
• Target: Adenosine A1 receptor
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: NDHFRCQPAPPIDED
• RRID: AB_10752439
• MW: 36.5; 29.4; 24.1
• Tested applications: ELISA, FC, WB
• Dilution range: ELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 10ug/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave bands at approx 30kDa and 20kDa in Human Brain (Cerebellum) lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 36.5kDa according to NP_000665.1 and NP_001041695.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti ADORA1 antibody, anti adenosine A1 receptor a
• Note: For research use only
• Entrez: 134

Western blot analysis of HEK293 cell using ADORA1 antibody.

Flow cytometric analysis of HeLa cells using ADORA1 antibody.