Acylglycerol kinase Antibody

SKU: orb19591

Description

Goat polyclonal antibody to AGK

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:16000, WB: 1-3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 50kDa band observed in Human Brain (Substantia nigra, Hippocampus) lysates (calculated MW of 47.1kDa according to NP_060708.1). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Acylglycerol kinase
• Molecular Weight: 47.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti acylglycerol kinase antibody, anti FLJ10842 antibody, anti MULK antibody, anti multi-substrate lipid kinase antibody, anti multiple substrate lipid kinase antibody, anti AGK antibody

orb19591 (1ug/ml) staining of Human Brain (Substantia nigra) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Acylglycerol kinase

NCBI Gene ID

55750