ACPP/PAP Antibody

SKU: orb20559

Description

Goat polyclonal to ACPP

Research Area

Cancer, Epigenetics

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: ELISA: 1:64000, WB: 0.3-1 μg/ml, IHC-P: 3-5µg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Rat
• Application Notes:
  IHC: In paraffin embedded Human Prostate shows cytoplasm staining in some secretory cells of the gland. Recommended concentration, 3-5μg/ml.WB: Approx 50kDa band observed in Human Prostate lysates (calculated MW of 44.6kDa according to NP_001090.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Patel et al, ,Clin Chem. 1986 Oct;32(10):1832-5. PMID: 3530530). Recommended concentration: 0.3-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ACPP / PAP
• Protein Sequence: YGIHKQKEKSRLQ
• Molecular Weight: 44.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ACPP antibody, anti acid phosphatase, prostate antibody, anti ACP-3 antibody, anti ACP3 antibody, anti PAP antibody, anti prostatic acid phosphotase antibody

Western blot analysis of human prostate lysate (35µg protein in RIPA buffer) using ACPP antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded human prostate tissue using ACPP antibody

Quick Database Links

Gene Symbol

ACPP / PAP

NCBI Gene ID

55