Goat anti-ACPP / PAP Antibody

• Catalog Number: orb20559
• Category: Antibodies
• Description: Goat polyclonal to ACPP
• Target: ACPP / PAP
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: YGIHKQKEKSRLQ
• RRID: AB_10751317
• MW: 44.6
• Tested applications: ELISA, IF, IHC, WB
• Dilution range: ELISA: 1:64000, WB: 0.3-1 μg/ml, IHC-P: 3-5µg/ml
• Application notes: IHC: In paraffin embedded Human Prostate shows cytoplasm staining in some secretory cells of the gland. Recommended concentration, 3-5μg/ml.WB: Approx 50kDa band observed in Human Prostate lysates (calculated MW of 44.6kDa according to NP_001090.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Patel et al, ,Clin Chem. 1986 Oct;32(10):1832-5. PMID: 3530530). Recommended concentration: 0.3-1 μg/ml.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti ACPP antibody, anti acid phosphatase, prostat
• Note: For research use only
• Entrez: 55

Western blot analysis of human prostate lysate (35µg protein in RIPA buffer) using ACPP antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded human prostate tissue using ACPP antibody