Goat anti-ACOX2 (Human) Antibody

• Catalog Number: orb19435
• Category: Antibodies
• Description: Goat polyclonal antibody to ACOX2
• Target: ACOX2 (Human)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QWAQKSPTNTQENP
• RRID: AB_10748403
• MW: 76.8
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:4000, WB: 0.5-1.5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 75kDa band observed in Human Liver lysates (calculated MW of 76.8kDa according to NP_003491.1). Recommended concentration: 0.5-1.5 μg/ml. An additional band of 37kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti ACOX2 antibody, anti acyl-Coenzyme A oxidase
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 8309
• Expiration Date: 12 months from date of receipt.

orb19435 (0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.