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Goat anti-ACOX2 (Human) Antibody

SKU: orb19435

Description

Goat polyclonal antibody to ACOX2

Images & Validation

Tested ApplicationsELISA, WB
Dilution rangeELISA: 1:4000, WB: 0.5-1.5 μg/ml
ReactivityHuman
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 75kDa band observed in Human Liver lysates (calculated MW of 76.8kDa according to NP_003491.1). Recommended concentration: 0.5-1.5 μg/ml. An additional band of 37kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Key Properties

HostGoat
ClonalityPolyclonal
TargetACOX2 (Human)
Molecular Weight76.8
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
DisclaimerFor research use only

Alternative Names

anti ACOX2 antibody, anti acyl-Coenzyme A oxidase 2, branched chain antibody, anti BCOX antibody, anti BRCACOX antibody, anti BRCOX antibody, anti THCCox antibody, anti Peroxisomal branched chain acyl-CoA oxidase antibody, anti THCA-CoA oxidase antibody, anti Trihydroxycoprostanoyl-CoA oxidase antibody
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Goat anti-ACOX2 (Human) Antibody

orb19435 (0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Goat anti-ACOX2 (Human) Antibody (orb19435)

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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