Aconitase 2 Antibody

SKU: orb20462

Description

Goat polyclonal antibody to ACO2

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.01-0.03 μg/ml, IHC-P: 3-5µg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  IHC: In paraffin embedded Human Breast shows mitochondrial staining in the epithelial cells of the ducts. Recommended concentration, 3-5μg/ml.WB: Approx 90kDa band observed in Human Heart lysates (calculated MW of 85.4kDa according to NP_001089.1). Recommended concentration: 0.01-0.03 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Aconitase 2
• Protein Sequence: QHVDVSPTSQRLQ
• Molecular Weight: 85.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti aconitase 2, mitochondrial antibody, anti ACONM antibody, anti MGC20605 antibody, anti MGC33908 antibody, anti ACO2 antibody

Western blot analysis of heart lysate (35µg protein in RIPA buffer) using Aconitase 2 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded human breast tissue using Aconitase 2 antibody

Quick Database Links

Gene Symbol

Aconitase 2

NCBI Gene ID

50