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ABCE1/RNase L Antibody

SKU: orb20024

Description

Goat polyclonal antibody to ABCE1

Images & Validation

Tested ApplicationsELISA, FC, IF, WB
Dilution RangeELISA: 1:32000, WB: 0.2 μg/ml
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx. 80kDa band in Human Spleen lysates after 0.2 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.3kDa according to NP_002931.2. The ~80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

HostGoat
ClonalityPolyclonal
TargetABCE1 / RNase L
Protein SequenceNHDKCKPKKCRQEC
Molecular Weight67.3
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti ABCE1 antibody, anti ATP-binding cassette, sub-family E (OABP), member 1 antibody, anti ABC38 antibody, anti OABP antibody, anti RLI antibody, anti RNASEL1 antibody, anti RNASELI antibody, anti RNS4I antibody, anti ATP-binding cassette, sub-family E, member 1 antibody, anti RNase L inhibitor antibody, anti ribonuclease L (2\1,5\1-oligoisoadenylate synthetase-dependent) inhibitor antibody

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NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

ABCE1/RNase L Antibody (orb20024)

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Available Sizes

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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