Goat anti-ABCB5 Antibody

• Catalog Number: orb19707
• Category: Antibodies
• Description: Goat polyclonal antibody to ABCB5
• Target: ABCB5
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QTQHRNTSKKAQ
• RRID: AB_10751730
• MW: 89.8; 139
• Tested applications: ELISA, FC, WB
• Dilution range: ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 10ug/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 80kDa band observed in lysates of A431 (calculated MW of 89.8kDa according to NP_848654.3). Recommended concentration: 0.03-0.1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti ATP-binding cassette, sub-family B (MDR/TAP),
• Research Area: Cancer, Epigenetics
• Note: For research use only
• Entrez: 340273

orb19707 (0.03ug/ml) staining of A431 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.