ABCA2 Antibody

SKU: orb19878

Description

Goat polyclonal antibody to ABCA2

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution range: ELISA: 1:2000, WB: 0.3 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Preliminary experiments gave an approx 35kDa band in Human Brain (Amygdala, Frontal Cortex, Hippocampus and Substantia Nigra) lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 270kDa according to NP_001597.2 and of 273kDa according to.NP_997698.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ABCA2
• Molecular Weight: 270; 273
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti ABCA2 antibody, anti ATP-binding cassette, sub-family A (ABC1), member 2 antibody, anti ABC2 antibody, anti MGC129761 antibody, anti ATP-binding cassette, sub-family A, member 2 antibody, anti OTTHUMP00000064733 antibody

Quick Database Links

Gene Symbol

ABCA2

NCBI Gene ID

20