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| Catalog Number | orb19878 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to ABCA2 |
| Target | ABCA2 |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Protein Sequence | KKQSDNLEQQETEP |
| RRID | AB_10746373 |
| MW | 270; 273 |
| Tested applications | ELISA, IHC |
| Dilution range | ELISA: 1:2000, WB: 0.3 μg/ml, IHC-P: 3.75 μg/ml |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Preliminary experiments gave an approx 35kDa band in Human Brain (Amygdala, Frontal Cortex, Hippocampus and Substantia Nigra) lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 270kDa according to NP_001597.2 and of 273kDa according to.NP_997698.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti ABCA2 antibody, anti ATP-binding cassette, su Read more... |
| Research Area | Epigenetics |
| Note | For research use only |
| Entrez | 20 |
| Expiration Date | 12 months from date of receipt. |
WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rat, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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