A4GNT/alpha4GNT Antibody

SKU: orb20417

Description

Goat polyclonal antibody to A4GNT

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:8000, WB: 1-3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 40kDa band observed in lysates of cell line K562 (calculated MW of 39.5kDa according to NP_057245.1). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: A4GNT / alpha4GNT
• Molecular Weight: 39.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti A4GNT antibody, anti alpha-1,4-N-acetylglucosaminyltransferase antibody, anti MGC149493 antibody, anti alpha4GnT antibody

orb20417 (1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

A4GNT / alpha4GNT

NCBI Gene ID

51146

References

Weihui Fu ∙ Jun Fan ∙ Tianyun Peng ∙ Peng Sun ∙ Songhua Yuan ∙ Cuisong Zhu ∙ Lonfei Ding ∙ Linxia Zhang ∙ Chen Zhao ∙ Shuye Zhang Trefoil factor 2 Interacts with A4GNT to Regulate Inflammation to Protect against Pneumonia during Influenza Virus Infection iScience, (2025)

PubMed: