A4GNT/alpha4GNT Antibody

• Catalog Number: orb20417
• Category: Antibodies
• Description: Goat polyclonal antibody to A4GNT
• Target: A4GNT / alpha4GNT
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: PRTYRDLIKGPEGS
• RRID: AB_10749796
• MW: 39.5
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:8000, WB: 1-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 40kDa band observed in lysates of cell line K562 (calculated MW of 39.5kDa according to NP_057245.1). Recommended concentration: 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti A4GNT antibody, anti alpha-1,4-N-acetylglucos
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 51146

References

Weihui Fu ∙ Jun Fan ∙ Tianyun Peng ∙ Peng Sun ∙ Songhua Yuan ∙ Cuisong Zhu ∙ Lonfei Ding ∙ Linxia Zhang ∙ Chen Zhao ∙ Shuye Zhang Trefoil factor 2 Interacts with A4GNT to Regulate Inflammation to Protect against Pneumonia during Influenza Virus Infection iScience, (2025)

PubMed:

orb20417 (1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.